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Home » Sexual Health » Fertility

One Step® 20 x Ovulation Tests - Highly Sensitive Ovulation/Fertility Tests - 20mIU/mL Sensitive Tests


One Step® 20 x Ovulation Tests - Highly Sensitive Ovulation/Fertility Tests - 20mIU/mL Sensitive Tests

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A pregnancy begins with conception. A child is conceived when the male sperm successfully fertilises the female egg. Successful fertilisation is most likely during a 24-hour period following the L.H. (Luteinising Hormone) surge. Since this window is only open once a month and for a short period of time, being able to predict the L.H (Luteinising Hormone) surge is very helpful when trying to become pregnant.

We have selected these ovulation tests for their price, accuracy and reliability. Both these tests look for the presence of L.H. (Luteinising Hormone) in urine.

How accurate is this test?

This test is greater than 99% accurate in laboratory testing.

How does a home ovulation test work?

When a woman is about to ovulate, her body releases a large amount of a hormone called L.H. (Luteinising Hormone). L.H. is always present in your urine but the levels increase (surge) in the middle of your cycle, causing you to release an egg from the ovary, this process is called ovulation. If the L.H. surge is present, ovulation is likely to occur in the next 24 to 48 hours.

These ovulation tests detect the L.H. surge by measuring the amount of L.H. in urine.

Once I receive a positive result, when is the best time to have intercourse?

Ovulation usually follows the start of the L.H. surge within 24-48 hours. This is the time when you are at your most fertile, therefore you should have intercourse during this time.

What happens if after using the 5 tests provided I have still not detected my L.H. surge?

Most women with regular cycles will detect their L.H. Surge in 5 days or under and so 5 tests are sufficient, however, if you have irregular cycles you may need to test for longer and may require additional ovulation tests to detect your L.H. Surge

I have recently come off the contraceptive pill will this affect my result?

As the pill disrupts your natural hormone balance it may take a few months for your periods to return to normal. You may want to wait until you have had 2 normal periods before starting to use this ovulation test. Please be aware that pregnancy and breast feeding could affect the results of this test.

Will alcohol or other common medications affect the results of this test?

No, but you should consult your doctor if you are taking any hormonal treatments.

Can I use the ovulation test for contraception?

No, the test is not designed to prevent contraception. As sperm can survive for 72 hours you might still become pregnant if you had intercourse before you detected your L.H. Surge

I had a positive result for the L.H. Surge and had sexual intercourse during this period but I have still not become pregnant. Why?

There are many factors that can affect your fertility. It can take many healthy couples months to achieve pregnancy. You may need to use these tests for a few months before pregnancy occurs. If after 6 months of trying you have still not become pregnant, you should visit your doctor for advice. For more information and tips on trying for a baby

Ovulation Strip Test – CE MARKED 0123


Ovulation is the release of an egg from the ovary. The egg then passes into the fallopian tube where it is ready to be fertilized. A child is conceived when the male sperm successfully fertilizes the female egg.  When a woman is about to ovulate, her body releases a large amount of a hormone called L.H. (Luteinising Hormone). L.H. is always present in your urine but the levels increase (surge) in the middle of your cycle, causing you to release an egg from the ovary.

Intended Use:

The One Step Ovulation Test is an immunochromatographic in-vitro assay for the qualitative and semi-quantitative determination of the human luteinizing hormone (LH) in urine to predict the time of ovulation in women. One Step detects the sharp increase in LH concentration in urine, the so called “LH surge” which precedes ovulation.  Conception is most likely to occur within 36 hours following the LH surge.


Each pouch contains one immunochromatographic test. Each test contains a membrane with an anti-LH antibodies and conjugates, in a stabilizing matrix containing proteins and sodium azide.

Storage and Stability:

Store below 30°C; do not freeze.


One Step is for in vitro diagnostic use only.

Elevated concentrations of Human Chorionic Gonadtropin (HCG) interfere with LH testing. Do not test samples from pregnant women and people with pathologic conditions causing higher HCG levels

One Step is not designed to prevent contraception. As sperm can survive for 72 hours you might still become pregnant if you had intercourse before you detected your L.H. surge.

When to Begin Testing:

First, you must determine the length of your menstrual cycle.  This is the number of days from the first day of your menstrual bleeding to the day before your next bleeding begins again, count the first day of bleeding as day 1. Calculate what the usual length of your menstrual cycle has been the last few months. Once you have worked out the length of your cycle refer to the chart to determine on which day of your menstrual cycle you should begin testing.

Your Cycle Length

Start To Test On

21 days

Day 6

22 days

Day 6

23 days

Day 7

24 days

Day 7

25 days

Day 8

26 days

Day 9

27 days

Day 10

28 days

Day 11

29 days

Day 12

30 days

Day 13

31 days

Day 14

32 days

Day 15

33 days

Day 16

34 days

Day 17

35 days

Day 18

36 days

Day 19

37 days

Day 20

38 days

Day 21

39 days

Day 22

40 days

Day 23


For example, if your usual cycle is 28 days.  If your first day of last menstruation is 7th, you should begin testing on day 18th.

Example Calendar







































(   )–First day of your last period

<  >– Begin testing with the One Step Ovulation Test (Urine).


If your cycle is shorter than 21 days or longer than 40 days, consult your doctor. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform 1 test each day until the LH surge has been detected.

Specimen Collection:

Once you have identified what day you should begin testing you should then begin to collect your urine on a daily basis.

  1. Do not use first morning urine samples as LH is synthesized in your body early in the morning. It will not show up in your urine until later in the day.
  2.  The best time to collect your urine is between 10am- 8pm. Pick a regular time that suits you best.
  3. Collect urine at about the same time each day. Reduce liquid intake about 2 hours before collecting your urine as a diluted urine sample can prevent the test from detecting LH surge.

Before You Begin:

1 Read the instructions thoroughly before you begin.

2. Do not open the foil pouch until you are ready to begin the test.

3. Make sure you have a watch, clock or timer ready.

4. Allow urine samples and test kit to reach room temperature before testing (approx 20 mins).

Test Procedure:

1.Determine the day to begin testing.

2.To begin testing, open the sealed pouch and remove the strip. Do not remove the strip until you are ready to begin testing.

3. With the arrows pointing downwards towards the urine, place the test strip vertically (straight) into the urine sample, for at least 10 seconds. DO NOT allow the urine to go above the MAX (maximum) level line.

4.Remove the strip from the urine and place on a clean, dry surface. For best results you should read the results at 8 minutes.

5.NOTE Do not interpret One Step results after more than five minutes. To avoid confusion, discard the test after registering the test result at 10 minutes.

Interpretation of Results:

After each test, you must decide if you are having a L.H. surge.

To determine your result you must compare the colour intensity of the test band to the control band. The control band is used to compare the test band against and also confirms that you have completed the test correctly.

Positive for L.H. surge

If two colour bands are visible and the test band is of equal or greater colour intensity (darker) than the control band, this is a positive result and a good indication that the L.H. surge is occurring. You should ovulate within the next 24-36 hours. Sexual intercourse on the evening of the first positive test is advised.

Negative for L.H. surge

If two bands are visible but the test band is of a less intense colour (paler) than the control band or cannot be seen, this means the L.H. level is at or near its normal level and that the surge is not in progress. You should continue with daily testing.

Invalid result

If no control band appears within 5 minutes, the result is invalid and should be ignored. A visible control line is needed in all cases to confirm a proper test result. Repeat test with a new test kit.

ovulation test

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10 x One Step® Ovulation Tests - 20mIU/mL Sensitive Tests - £2.94

20 x One Step® Ovulation Tests - 20mIU/mL Sensitive Tests - £3.83 - Save £2.05

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30 x One Step® Ovulation Tests - 20mIU/mL Sensitive Tests - £5.68 - Save £3.14


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